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Chapter 25: Q23P (page 709)

To which kinds of analytes do these liquid chromatography detectors respond?

(a) ultraviolet

(b) refractive index

(c) evaporative light scattering

(d) charged aerosol

(e) electrochemical

(f) fluorescence

(g) nitrogen chemiluminescence

(h) conductivity

Short Answer

Expert verified

The part (a), part (b), part (c), part (d), part (e), part (f), part (g), part (h) is

  1. Wavelengths below 210
  2. Its detection limit is about poorer than that of the ultraviolet detector
  3. significantly less volatile than the mobile phase
  4. Itsnearly equal response to equal masses of non-volatile analytes
  5. Chromatography detector responds to analytes that can be oxidized or reduced
  6. Chromatography detector responds only to the few analytes that fluoresce
  7. Chromatography detector responds to the molecules with N atom
  8. Chromatography detector responds ionic or ionizable compounds

Step by step solution

01

Ultraviolet

Part (a)

The ultraviolet liquid chromatography detector is selective for compounds with wavelengths above 210 nm and is universal for compounds with wavelengths below 210.

02

Refractive index

Part (b)

The refractive index liquid chromatography detector responds to almost every solute, but its detection limit is about poorer than that of the ultraviolet detector.

03

Evaporative light scattering

Part (c)

The evaporative light scattering liquid chromatography detector responds to any analyte that is significantly less volatile than the mobile phase.

04

Charged aerosol

Part (d)

The charged aerosol liquid chromatography detector is almost universal detector with nearly equal response to equal masses of non-volatile analytes.

05

Electrochemical

Part (e)

The electrochemical liquid chromatography detector responds to analytes that can be oxidized or reduced (for example it can be phenols, aromatic amines).

06

Fluorescence

Part (f)

The fluorescence liquid chromatography detector responds only to the few analytes that fluoresce.

07

Nitrogen chemiluminescence

Part (g)

The nitrogen chemiluminescence liquid chromatography detector responds to the molecules with N atom.

08

Conductivity

Part (h)

The conductivity liquid chromatography detector responds ionic or ionizable compounds.

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Most popular questions from this chapter

A reversed-phase separation of a reaction mixture calls for isocratic elution with 48%methanol 52%water. If you want to change the procedure to use acetonitrile/water, what is a good starting percentage of acetonitrile to try?

  1. Use equation 25-1to estimate the length of a column required to achieve1.0脳104plates if the stationary phase particles size is10.5,5.0,3.0,or1.5渭尘

  2. If the retention time was 20mins on the 10.0渭尘 particle size column, what is the retention time on the 5.0,3.0,or1.5渭尘columns from part (a)? Assume that flow rate is constant for all columns.

  3. Use equation25-2to estimate the pressure of the column in (a) given that the pressure of the10.0渭尘column was4.4Mpa

  4. If the flow rate was2.0mL/min , what is the baseline width for the peaks on 10.5,5.0,3.0,or1.5渭尘columns form part (a)?

  5. Which of these column configurations would require a UHPLC instrument?

A known mixture of compounds A and B gave the following HPLC results:

A solution was prepared by mixing 12.49mgof Bplus 10.00mLof unknown containing just and diluting to 25.00mL. Peak areas of 5.97and 6.38were observed for AandB, respectively. Find the concentration of A(mg/mL)in the unknown.

Why are silica stationary phase height limited to operating in the pH range 2-8?why does the silica in figure25-8have improved stability at low pH?

HPLC peak should generally not have an asymmetry factor, B/A in figure 23-14,outside the range0.9-1.5

  1. Sketch the shape of a peak with an asymmetry of 1.8
  2. What might you do to correct the asymmetry?
See all solutions

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