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Chapter 26: Q31P (page 748)
What is the principal source of zone broadening in ideal capillary electrophoresis?
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State three different methods to reduce electroosmotic flow. Why does the direction of electroosmotic flow change when a silica capillary is washed with a cationic surfactant?
Electroosmotic velocities of buffered solutions are shown for a bare silica capillary and one with aminopropyl groups (silica鈥擲iOCH2CH2CH2NH2) covalently attached to the wall. A positive sign means that flow is toward the cathode. Explain the signs and relative magnitudes of the velocities.
d) has lower mobility than . Explain why lithium phosphate can be used at higher electric field strength than sodium phosphate to generate the same current. What is the advantage of higher field strength for this separation?
Low iron concentration (as low as 0.02nM) in the open ocean limits phytoplankton growth. Preconcentration is required to determine such low concentrations. Tracefrom a large volume of seawater is concentrated onto achelating resin column. The column is then rinsed with 10mLof 1.5M high-purity water and eluted withofhigh-purity.
(a) For each sample, seawater is passed through the column for 17hours at . How much is the concentration of in the 10mLof eluate increased by this preconcentration procedure?
(b) What is the concentration of in the seawater when 57 nm is found in the nitric acid eluate?
(c) Reagent-grade concentrated nitric acid is 15.7 M and contains iron. What would be the apparent concentration of Fe (nM) in a seawater blank if reagent-grade acid were used to prepare the 1.5M eluent?
Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by molecular exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 3 0.65 5 14.3 mL)Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at , findfor transferrin.
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