91Ó°ÊÓ

Molecule mass is a molecular weight that is equal to the sum of all the atoms contained in a molecule.

(a)

Here we will find $V_0$for the 25-nm-pore-size column

The starting point of the curve for 25nm pores which rises vertically at the left would be 11 and end point would be around 5.5 so the ${\mathrm{V}}_0=5.5\mathrm{ml}$ml.

The smallest molecular mass of molecules excluded from this column -${10}^6$ Da.

(b)

Here we will find the molecular mass of molecules eluted at 9.7 mL from the 12.5-nm column.

Look at the Figure 26-15 and find that the molecular mass is${10}^4$ Da.

(c)

Here we will find the largest molecular mass that can freely enter the 45-nm pores

Considering that${\mathrm{V}}_{\mathrm{m}}$ is the volume at which the curves drop vertically at the right - the largest molecular mass would be -${10}^4$ Da.