Chapter 26: Q1TY (page 717)
Find the milliequivalents ofreleased if100mLofwere loaded on a cation exchange column in theform.
Short Answer
The milli equivalents of that will be replaced by 100mL is
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Chapter 26: Q1TY (page 717)
Find the milliequivalents ofreleased if100mLofwere loaded on a cation exchange column in theform.
The milli equivalents of that will be replaced by 100mL is
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The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.
(a) How many moles of analyte are present in asolution that occupies 1.0% of the length of acapillary?(b) What voltage is required to inject this many moles into a capillary in 4.0 s if the sample has 1/10 of the conductivity of background electrolyte, and the sample concentration is
A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?
(a) A particular solution in a particular capillary has an electroosmotic mobility of and 8.1 . How long will it take a neutral solute to travel 52 cm from the injector to the detector if 27 kV is applied across the 62-cm-long capillary tube at pH 2? At pH 12?(b) An analyte anion has an electrophoretic mobility of How long will it take to reach the detector at pH 2?At pH 12?
State three different methods to reduce electroosmotic flow. Why does the direction of electroosmotic flow change when a silica capillary is washed with a cationic surfactant?
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