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Question: Describe the appearance of a Drosophila embryo, in which the knirps gene protein-coding sequence has been replaced by lacZ, following incubation in a solution of X-gal

Short Answer

Expert verified

After incubation in the X-gal solution, the band would appear blue stained because of the action of

β-galactosidase on X-gal.

Step by step solution

01

Gene

A functional unit of heredity is a gene. A gene is a nucleotide sequence in the genome that codes for a functional molecule. Various species have different numbers of genes.

02

Appearance of a Drosophila embryo, in which the knirps gene protein-coding sequence has been replaced by lacZ

The segmentation genes in Drosophila determine the correct sequence, number, and polarity of the embryo's body segments. The knirps gene acts as a gap gene during embryogenesis, controlling the expression of segmentation genes. The knirps gene regulates segmentation in the embryo's abdominal region.

03

Explanation of knirps gene protein-coding sequence which is replaced by lacZ, following incubation in a solution of X-gal

The knirps gene is replaced with lacZ, as stated in the statement. It's a reporter gene, and the embryo would form with -galactosidase expressed. The pattern of Drosophila can be investigated by staining -galactosidase. The knirps mRNA is expressed in the posterior side of the Drosophila embryo at the middle. As a result of the action of -galactosidase on X-gal, the band would look blue-stained following incubation in the X-gal solution.

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