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Chapter 24: Q11P (page 876)

How is the melting curve of duplex DNA affected by decreasing the ionic strength of the solution?

Short Answer

Expert verified

The Tm value of the melting curve of duplex DNA decreases as the ionic strength of the solution decreases.

Step by step solution

01

The melting curve of DNA.

The denaturation features of DNA are shown by the melting curve of DNA, which is based on nabsorption at a particular wavelength. Melting temperature,Tm, is the specific heatat which halves of the DNA are denatured.

02

The effect of the melting curve of duplex DNA.

The electrons of phosphate groups are much more exposed to one other at lower ionic strength. As a result, they reject each other even more. Phosphate group repulsion destabilizes the double helix, lowering the melting curve of duplex DNA.

Hence, the Tm value of the melting curve of duplex DNA decreases as the ionic strength of the solution decreases.

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