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L-ascorbic acid is another name for ascorbic acid, which is both a vitamin C and an antioxidant. Galactose dehydrogenase is an oxidoreductase enzyme that belongs to the oxidoreductase family. This has a particularly strong effect on the CH-OH donor group.

TheReaction 8 in this pathway uses the enzyme galactose dehydrogenase. L-galactose is transformed to L-galactono-1, 4-lactone in reaction 8. Galactose undergoes a reduction reaction throughout this phase.

GDP-D-mannose-3, 5-epimerase is an isomerase that plays a role in the metabolism of ascorbate and aldarate. GDP-D-mannose is changed to GDP-L-galactose in Reaction 5. An isomerase reaction occurs here.

The enzyme phosphoglucose isomerase transforms D-glucose-6-phosphate to D-fructose-6-phosphate. This reaction occurs in Reaction-1 of this biosynthetic pathway.

The interconversion of fructose-6-phosphate to mannose-6-phosphate is facilitated by the enzyme phosphomannose isomerase. Reaction 2 continues this reaction.

The catalytic enzyme phosphomannose mutase catalyses Reaction 3 in the hypothesised ascorbic acid biosynthesis pathway, which converts D-mannose phosphate-6-phosphate to D-mannose-1-phosphate.

Thus, in Reaction 8, the enzyme galactose is utilized. In Reaction 5, the enzyme GDP-D-mannose-3,5-epimerase is activated. In Reaction 1, the enzyme phosphoglucose isomeraseis used. In Reaction 2, the enzyme phosphomannose isomerase is introduced. In Reaction 3, the enzyme phosphomannose mutase is involved.