Q9. At what concentration of S (expr... [FREE SOLUTION]

Chapter 12: Q9. (page 398)

At what concentration of $S$ (expressed as a multiple of $K_{M}$ ) will $v_{0} = 0.95 V_{\max}$ ?

Short Answer

Expert verified

The concentration of $S$ at which $v_{0} = 0.95 V_{\max}$ is $19 K_{M}$ .

Step by step solution

Introduction

The substrate or reactant is the molecule that binds to the enzyme, and the enzyme converts it into the product. $v_{0}$ is the enzyme initial velocity. It stimulates the formation of the product from the reactant. $V_{\max}$ is the maximum rate of the reaction.

Michaelis-Menten equation

According to Michaelis-Menten equation,

$v_{0} = \frac{V_{\max} [S]}{K_{M} + [S]}$

where

$v_{0}$ is the initial velocity.

$V_{\max}$ is the maximum velocity.

$[S]$ is the concentration of the substrate

$K_{M}$ is the Michaelis-Menten constant.

Calculation of substrate concentration

On substituting the values in the equation,

$0.95 V_{\max} = \frac{V_{\max} [S]}{K_{M} + [S]} 0.95 [S] + 0.95 K_{M} = [S] 0.05 [S] = 0.95 K_{M} [S] = \frac{0.95 K_{M}}{0.05} [S] = 19 K_{M}$

Thus, the concentration of the substrate is 19K_M.

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Substrate concentration (渭惭)	Reaction velocity (渭惭 s鈦宦�)
1	5
100	50

91影视

At what concentration of $S$ (expressed as a multiple of $K_{M}$ ) will $v_{0} = 0.95 V_{\max}$ ?

Short Answer

Step by step solution

Introduction

Michaelis-Menten equation

Calculation of substrate concentration

One App. One Place for Learning.

Most popular questions from this chapter

Recommended explanations on Biology Textbooks

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91影视

At what concentration of S (expressed as a multiple of KM ) will v0=0.95Vmax?

Short Answer

Step by step solution

Introduction

Michaelis-Menten equation

Calculation of substrate concentration

One App. One Place for Learning.

Most popular questions from this chapter

Recommended explanations on Biology Textbooks

Responding to Change

Communicable Diseases

Ecology

Bioenergetics

Chemistry of Life

Biological Structures

Study anywhere. Anytime. Across all devices.

At what concentration of $S$ (expressed as a multiple of $K_{M}$ ) will $v_{0} = 0.95 V_{\max}$ ?