Chapter 11: Q26 P (page 359)

Predict the effect on lysozyme鈥檚 activity of mutating Glu 35 to Asp and Asp 52 to Glu.

Short Answer

Expert verified

The catalytic activity of lysozyme would be lost.

Step by step solution

Lysozyme

Animals generate the antimicrobial enzyme lysozyme, also known as muramidase or N-acetylmuramideglycanhydrolase, which is a component of the innate immune system.

Explanation of the effect on lysozyme activity

If Glu and Asp were to switch places, lysozyme's catalytic activity would decrease. The pKa of the relevant residues determines the catalysis process, with Glu being protonated and Asp 52 being deprotonated. Due to its slightly higher pKa than aspartic acid, glutamic acid would be protonated in position 52 , preventing it from forming a covalent connection with the oxonium ion during catalysis.

Hence, lysozyme would lose catalytic activity.

Unlock Step-by-Step Solutions & Ace Your Exams!

Full Textbook Solutions
Get detailed explanations and key concepts
Unlimited Al creation
Al flashcards, explanations, exams and more...
Ads-free access
To over 500 millions flashcards
Money-back guarantee
We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91影视!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Recommended explanations on Biology Textbooks

View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

91影视

Predict the effect on lysozyme鈥檚 activity of mutating Glu 35 to Asp and Asp 52 to Glu.

Short Answer

Step by step solution

Lysozyme

Explanation of the effect on lysozyme activity

One App. One Place for Learning.

Most popular questions from this chapter

Recommended explanations on Biology Textbooks

Biological Molecules

Ecological Levels

Microbiology

Biological Organisms

Organ Systems

Responding to Change

Study anywhere. Anytime. Across all devices.