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Chapter 11: Q12P (page 359)

Question: Explain why RNase A cannot catalyze the hydrolysis of DNA

Short Answer

Expert verified

Answer

DNA does not have the 2鈥�-OH group which is required for hydrolyzing into intermediates like 2鈥�,3鈥�-cyclic nucleotides

Step by step solution

01

Introduction of RNase A

RNase A is a digestive enzyme which mediates acid-base catalysis. It is secreted by the pancreas in the small intestine and its function is to hydrolyze RNA to its component nucleotides

02

Explanation for the inability of RNase A to catalyze the hydrolysis of DNA.

DNA does not have the 2鈥�-OH group which is required for hydrolyzing into intermediates like 2鈥�,3鈥�-cyclic nucleotides.Whereas RNA has the 2鈥�-OH groups which help RNase in catalyzing the hydrolysis of RNA.

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