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Chapter 16: 16-6TYU (page 334)

E.coli grown on a 15N medium is transferred to a 14N medium and allowed to grow for two more generations (two rounds of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment?

  1. one high-density and one low-density band
  2. one intermediate density band
  3. one high density and one intermediate-density band
  4. one low-density and one intermediate-density band

Short Answer

Expert verified
  1. The option 鈥渙ne high-density and one low-density band鈥� is false.
  2. The option 鈥渙ne intermediate density band鈥� is false.
  3. The option 鈥渙ne high density and one intermediate-density band鈥� is false.
  4. The option 鈥渙ne low-density and one intermediate-density band鈥� is true.

Step by step solution

01

DNA

DNA is the genetic material that is found in most living organisms, including bacteria. It carries the genetic information which passes from one generation to the other generation.

02

Explanation of option (A)

The observation of one high density and low-density band is not seen since the experiment was conducted for two generations in the 14N medium.

After two rounds of DNA replication, no high-density band formation is observed because the E.coli was moved to the14N medium.

Therefore, the given option is false.

03

Explanation of option (B)

The observation of one intermediate band is not the only result of the experiment since the bacteria is grown in two different types of medium containing 15N and 14N.

Thus, there must be the formation of two different bands having varying densities.

Therefore, the given option is false.

04

Explanation of option (C)

The observation of one high density and one intermediate-density band is not the result of this experiment. Since the E.coli grown on the medium containing 15N is transferred to the 14N.

After this transfer, two round of replication is carried out only in the 14N medium.

Thus, the observation of a high-density band that corresponds to the 15N medium is not seen.

Therefore, the given option is false.

05

Explanation of option (D)

The transfer of E.coli from the 15N is transferred to the 14N, making the bacterium adapted to the 14N. Since the bacterium is grown initially in the15N heavier than the 14N, it produces the one intermediate band, not the high-density band.

The two rounds of replication are taking place in the14N that forms the low density and intermediate bands.

Therefore, the given option is true.

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Most popular questions from this chapter

What is the basis for the difference in how the leading and lagging strands of DNA molecules are synthesized?

  1. Origins of replication occur only at the 5鈥� end.
  2. Helicases and single-strand bindingproteins work at the 5鈥檈nd.
  3. DNA polymerase can join new nucleotides only to the 3鈥檈nd of a pre-existing strand, and the strands are antiparallel.
  4. DNA ligase works only in the 3鈥�5鈥� direction.

What two properties, one structural and one functional, distinguish heterochromatin from euchromatin?

This image shows DNA (grey) interacting with a computer-generated model of a TAL protein (multicolored), one of a family of proteins found only in a species of bacterium Xanthomonas. The bacterium uses proteins like this once to find specific gene sequences in cells of the organisms it infects, such as tomatoes, rice and citrus fruits. Given what you know about DNA structure and considering the image above, discuss how the TAL protein's structure suggests that it functions.

The elongation of the leading strand during DNA synthesis

  1. progress away from the replication fork.
  2. occurs in the 3鈥�鈫�5鈥�direction.
  3. produces Okazaki fragments.
  4. depends on the action of DNA polymerase.

Interphase chromosomes appear to be attached to the nuclear lamina and perhaps also the nuclear matrix. Describe these two structures. See Figure 6.9 and the associated text.
See all solutions

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