/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Problem 10 An experiment published in The A... [FREE SOLUTION] | 91Ó°ÊÓ

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An experiment published in The American Biology Teacher studied the efficacy of using \(95 \%\) ethanol or \(20 \%\) bleach as a disinfectant in removing bacterial and fungal contamination when culturing plant tissues. The experiment was repeated 15 times with each disinfectant, using eggplant as the plant tissue being cultured. \({ }^{8}\) Five cuttings per plant were placed on a petridish for each disinfectant and stored at \(25^{\circ} \mathrm{C}\) for 4 weeks. The observation reported was the number of uncontaminated eggplant cuttings after the 4 -week storage. Disinfectant \(95 \%\) Ethanol \(20 \%\) Bleach \begin{tabular}{lcc} \hline Mean & 3.73 & 4.80 \\ Variance & 2.78095 & .17143 \\ \(n\) & 15 & 15 \\ & Pooled variance 1.47619 & \end{tabular} a. Are you willing to assume that the underlying variances are equal? b. Using the information from part a, are you willing to conclude that there is a significant difference in the mean numbers of uncontaminated eggplants for the two disinfectants tested?

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And is there a significant difference in the mean numbers of uncontaminated eggplants for the two disinfectants tested?

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01

Hypothesis Test for Equality of Variances

First, we need to perform an F-test for the hypothesis that the variances are equal. The null hypothesis (\(H_0\)) is that the variances are equal, and the alternative hypothesis (\(H_1\)) is that the variances are not equal. \(H_0: \sigma^{2}_{1} = \sigma^{2}_{2}\) \(H_1: \sigma^{2}_{1} \neq \sigma^{2}_{2}\) To calculate the F-test statistic, divide the larger variance by the smaller variance: \(F = \frac{\text{larger variance}}{\text{smaller variance}}\) For our data, the larger variance is 2.78095 (from the 95% ethanol disinfectant), and the smaller variance is 0.17143 (from the 20% bleach disinfectant): \(F = \frac{2.78095}{0.17143} \approx 16.229\) Next, look up the critical values of F using the F-distribution table, with degrees of freedom (df) equal to the sample sizes minus 1: df1 = \(15-1=14\) df2 = \(15-1=14\) The critical values of F for 95% confidence level are 0.387 and 2.576. Since our test statistic (16.229) falls outside this range, we reject the null hypothesis. Therefore, we are not willing to assume that the underlying variances are equal.
02

t-test for Equality of Means

To determine if there is a significant difference in the mean numbers of uncontaminated eggplants for the two disinfectants tested, we will perform a t-test for independent samples with unequal variances (assuming unequal variances based on the F-test result from step 1). To perform the t-test, first state the null and alternative hypotheses: \(H_0: \mu_1 = \mu_2\) \(H_1: \mu_1 \neq \mu_2\) Next, calculate the pooled variance estimate (\(s^{2}_p\)), the test statistic (t), and the degrees of freedom (df): \(s^{2}_p = \frac{(n_1-1)s^{2}_{1} + (n_2-1)s^{2}_{2}}{n_1+n_2-2}\) \(t = \frac{(\bar{x}_{1} - \bar{x}_{2}) - (\mu_{1} - \mu_{2})}{\sqrt{\frac{s^{2}_{1}}{n_1} + \frac{s^{2}_{2}}{n_2}}}\) \(df = \frac{(\frac{s^{2}_{1}}{n_1} + \frac{s^{2}_{2}}{n_2})^{2}}{\frac{(\frac{s^{2}_{1}}{n_1})^{2}}{n_1-1} + \frac{(\frac{s^{2}_{2}}{n_2})^{2}}{n_2-1}}\) Using the given data: \(s^{2}_p = \frac{(15-1)(2.78095) + (15-1)(0.17143)}{15+15-2} \approx 1.47619\) \(t = \frac{(3.73 - 4.80) - (0)}{\sqrt{\frac{2.78095}{15} + \frac{0.17143}{15}}} \approx -4.939\) \(df = \frac{(\frac{2.78095}{15} + \frac{0.17143}{15})^{2}}{\frac{(\frac{2.78095}{15})^{2}}{14} + \frac{(\frac{0.17143}{15})^{2}}{14}} \approx 14.362\) Looking up the t-distribution table for 95% confidence level, with df = 14 (rounded down), we find the critical values to be -2.145 and 2.145. Since our test statistic (-4.939) falls outside this range, we reject the null hypothesis. Therefore, we are willing to conclude that there is a significant difference in the mean numbers of uncontaminated eggplants for the two disinfectants tested.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

F-test
An F-test is a statistical test used to determine if there are differences in variances between two groups. In simpler terms, it helps us assess if two samples have the same variability or spread in their data. For instance, when a researcher wishes to compare the variances in bacterial contamination between two disinfectants, an F-test can provide this insight.

The F-test involves calculating the F-statistic by dividing the larger variance by the smaller variance. This statistic is then compared to a critical value from the F-distribution table, which changes based on the sample sizes (degrees of freedom) and the confidence level. If the calculated F-statistic lies beyond the critical values, we reject the assumption that both variances are equal.

In the mentioned experiment, the calculated F-statistic was approximately 16.229, which exceeded the critical values, indicating inequality in variances.
t-test
A t-test is commonly used to determine if there is a significant difference in the means of two groups. This is particularly useful when comparing the effects of two treatments, such as different disinfectants on bacterial contamination. In simple terms, a t-test tells us if one method performs better than another in terms of averages.

In our experiment, since the F-test suggested that variances were unequal, a t-test assuming unequal variances was conducted. The procedure involves stating a null hypothesis that posits no difference in means, and an alternative hypothesis suggesting a disparity.

The t-statistic is then calculated based on the sample means, variances, and sizes. If this statistic is more extreme than the critical values from a t-distribution table, we can confidently say there is a significant difference in means. Here, the t-statistic was approximately -4.939, far beyond our critical range, leading us to conclude a significant difference exists between disinfectants' efficacy.
Critical Values
Critical values are essential benchmarks in hypothesis testing. They are the cutoff points that determine whether we reject a null hypothesis. These values depend on the chosen level of significance (usually 5% or 0.05 for a 95% confidence level) and the test being performed.

For F-tests, the critical values are determined from the F-distribution table based on the degrees of freedom for each sample. These values set boundaries at which we can assume no difference or a significant difference in variances exists. Similarly, in a t-test, critical values from a t-distribution help decide on the equality or difference of means.

In our case, the critical values for the F-test were 0.387 to 2.576, and for the t-test were -2.145 to 2.145. As the calculated statistics fell outside these ranges, hypotheses regarding variance and mean differences were rejected.
Independent Samples
Independent samples refer to groups that do not influence each other. Each sample is collected independently from the other. This concept is crucial in hypothesis testing because it affects the validity of statistical methods like t-tests and F-tests.

In independent samples, each data point from one sample does not pair with data points from another. For example, using different sets of plant cuttings to test 95% ethanol and 20% bleach means each disinfectant's outcomes are independently measured.

The tests assume that these samples are from normally distributed populations or that the sample sizes are large enough to justify normal approximation. Understanding that samples are independent helps ensure that the t-test or F-test results are reliable and can be generalized to broader contexts.

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Most popular questions from this chapter

Cholesterol The serum cholesterol Elevels of 50 subjects randomly selected from the L.A. Heart Data, data from an epidemiological heart disease study on Los Angeles County employees, follow. \(\begin{array}{llllllllll}148 & 304 & 300 & 240 & 368 & 139 & 203 & 249 & 265 & 229 \\ 303 & 315 & 174 & 209 & 253 & 169 & 170 & 254 & 212 & 255 \\ 262 & 284 & 275 & 229 & 261 & 239 & 254 & 222 & 273 & 299 \\ 278 & 227 & 220 & 260 & 221 & 247 & 178 & 204 & 250 & 256 \\ 305 & 225 & 306 & 184 & 242 & 282 & 311 & 271 & 276 & 248\end{array}\) a. Construct a histogram for the data. Are the data approximately mound- shaped? b. Use a \(t\) -distribution to construct a \(95 \%\) confidence interval for the average serum cholesterol levels for L.A. County employees.

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