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Ishikawa et al. (Journal of Bioscience and Bioengineering, 2012) studied the adhesion of various biofilms to solid surfaces for possible use in environmental technologies. Adhesion assay is conducted by measuring absorbance at \(\mathrm{A}_{590} .\) Suppose that for the bacterial strain Acinetobacter, five measurements gave readings of 2.69,5.76,2.67,1.62 and 4.12 dyne-cm \(^{2}\). Assume that the standard deviation is known to be 0.66 dyne-cm \(^{2}\). (a) Find a \(95 \%\) confidence interval for the mean adhesion. (b) If the scientists want the confidence interval to be no wider than 0.55 dyne-cm \(^{2}\), how many observations should they take?

Short Answer

Expert verified
(a) CI is (2.794, 3.950) dyne-cm². (b) At least 23 observations.

Step by step solution

01

Calculate the Sample Mean

To find the sample mean \( \bar{x} \), sum all the measurements and divide by the number of measurements. Here, we have five measurements: 2.69, 5.76, 2.67, 1.62, and 4.12.\[ \bar{x} = \frac{2.69 + 5.76 + 2.67 + 1.62 + 4.12}{5} = \frac{16.86}{5} = 3.372 \text{ dyne-cm}^2 \]
02

Determine the Z-Score for a 95% Confidence Level

For a 95% confidence interval, the Z-score you use is approximately 1.96 (this is based on the standard normal distribution table).
03

Calculate the Standard Error of the Mean (SEM)

The SEM is calculated as the standard deviation \( \sigma \) divided by the square root of the sample size \( n \). Here, \( \sigma = 0.66 \) and \( n = 5 \).\[ \text{SEM} = \frac{0.66}{\sqrt{5}} \approx 0.295 \]
04

Compute the Margin of Error

The margin of error (MoE) is the Z-score times the SEM.\[ \text{MoE} = 1.96 \times 0.295 \approx 0.578 \]
05

Calculate the 95% Confidence Interval

Using the sample mean and the margin of error, the 95% confidence interval is given by:\[ 3.372 \pm 0.578 \]This results in:\[ (3.372 - 0.578, 3.372 + 0.578) \approx (2.794, 3.950) \] dyne-cm².
06

Calculate Sample Size for Desired Confidence Width

To determine the sample size \( n \) needed for a confidence interval no wider than 0.55 dyne-cm², use the formula for margin of error \( \frac{Z \times \sigma}{\sqrt{n}} \le 0.275 \) (half of 0.55 as it represents each side).Solve for \( n \):\[ 1.96 \times \frac{0.66}{\sqrt{n}} \le 0.275 \]\[ \sqrt{n} \ge \frac{1.96 \times 0.66}{0.275} \approx 4.7 \]\[ n \ge 22.09 \]Since \( n \) must be a whole number, round up to \( n = 23 \).

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

Sample Mean
The sample mean is a measure of the average value in a set of data points. To find the sample mean, you add up all the data points and divide by the number of data points. It is essential in statistics as it provides a central value that the other data points are distributed around.
For instance, consider the measurements: 2.69, 5.76, 2.67, 1.62, and 4.12 dyne-cm². To calculate the sample mean, sum these values, which gives 16.86, and then divide by the number of measurements, which is 5:
  • \(\bar{x} = \frac{16.86}{5} = 3.372\ cm^2\text{ dyne-cm²}\bar{x} = \frac{16.86}{5} = 3.372\text{ dyne-cm²}\)

The sample mean serves as an estimate of the true mean of the population from which the sample was drawn. However, it's important to remember that the sample mean is affected by the sample size and the variability of the data.
Standard Deviation
Standard deviation is a statistic that measures the dispersion of a dataset relative to its mean and is calculated as the square root of the variance. A low standard deviation indicates that the data points tend to be close to the mean, whereas a high standard deviation indicates that the data points are spread out over a wider range of values.
In this problem, the standard deviation (\(\sigma\)) is given as 0.66. This statistic provides us an insight into the consistency of the measurements.
  • In simpler terms, it tells us how much the measurements vary from their average.

  • Knowing the standard deviation is crucial for calculating other statistical measures, like the standard error of the mean and confidence intervals.

Remember, it's always provided or previously calculated, so you should always have this value on hand before proceeding with related computations.
Margin of Error
The margin of error (MoE) gives us an understanding of the range within which the true population parameter is likely to fall. It is heavily relied on when constructing confidence intervals for a population parameter. The margin of error is calculated by multiplying the standard error of the mean (SEM) by the Z-score, which corresponds to the desired confidence level.
For a 95% confidence interval, the Z-score is typically 1.96. Given the standard deviation and sample size, we compute the SEM as:\[\text{SEM} = \frac{\sigma}{\sqrt{n}} = \frac{0.66}{\sqrt{5}} \approx 0.295\]Once the SEM is calculated, we find the margin of error:\[\text{MoE} = 1.96 \times 0.295 \approx 0.578\]
The margin of error allows us to estimate the distance from the sample mean that encapsulates the population mean with a certain level of confidence. This gives a more comprehensive picture, accounting for the variability and uncertainty in our data.
Sample Size Calculation
Determining the appropriate sample size is key to obtaining a desired level of precision in statistical estimates. In this context, scientists aimed to construct a confidence interval with a maximum width of 0.55 dyne-cm². To calculate the necessary sample size, we rearrange the formula for the margin of error and solve for sample size (\(n\)):\[\frac{Z \times \sigma}{\sqrt{n}} \le 0.275\]Here, 0.275 is half of the total desired width of 0.55, applicable to one tail of the distribution.
  • First, calculate the minimal required square root of the sample size:\[\sqrt{n} \ge \frac{1.96 \times 0.66}{0.275} \approx 4.7\]
  • Second, square this number to find the full sample size requirement:\[n \ge 22.09\]
  • Since sample size must be an integer, always round up to ensure reach of the desired precision. Thus, a sample size of 23 is appropriate.

Adequately calculating sample size helps ensure that the confidence interval is precise enough for meaningful interpretation.

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Most popular questions from this chapter

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