Chapter 4: Problem 1
What are the two most important characteristics of an enzyme?
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Chapter 4: Problem 1
What are the two most important characteristics of an enzyme?
These are the key concepts you need to understand to accurately answer the question.
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Compound 3 is a substrate for an enzyme that catalyzes the removal of the \(\mathrm{H}_{\mathrm{S}}\) proton exclusively. If compound 4 is used as the substrate, the deprotonation occurs mostly at \(\mathrm{H}_{\mathrm{S}}\), but a small amount of products are observed from removal of \(\mathrm{H}_{\mathrm{R}}\). This second reaction was shown to be catalyzed by the same enzyme as well. How could this be rationalized?
The following reaction is catalyzed by an enzyme at \(\mathrm{pH} 7\) and \(25^{\circ} \mathrm{C}\), whereas nonenzymatically, this reaction does not occur under these conditions. Explain how the enzyme can easily catalyze this reaction.
The \((3 S, 4 S)\)-isomer of deuterated compound \(\mathbf{1}\) is a substrate for a dephosphorylase that gives an antielimination to the \(Z\)-isomer (2). The corresponding (3R,4S)-isomer is not a substrate. How do you explain that?
Draw a mechanism for the formation of \(N^{5}, N^{10}-\) methylenetetrahydrofolate and the transfer of a hydroxymethyl group to uridylate.
\(\gamma\)-Aminobutyric acid (GABA) aminotransferase catalyzes a PLP-dependent conversion of GABA to succinic semialdehyde (SSA). a. Draw a mechanism for this reaction. b. Why is only one molecule of succinic semialdehyde formed in the absence of \(\alpha\)-ketoglutarate c. If the reaction were carried out in \({ }^{2} \mathrm{H}_{2}{ }^{18} \mathrm{O}\), what would the products be?
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