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91Ó°ÊÓ

Problem 1

What is the difference between an oncogene and a tumor suppressor gene?

Problem 1

What are the five histone proteins in eukaryotic cells? Which one is not part of the histone core around which DNA wraps to form chromatin?

Problem 1

What is the difference between a nucleoside and a nucleotide?

Problem 1

For each of the enzymes listed below, list the function of the enzyme and if it is found in prokaryotes, eukaryotes, or both. $$ \begin{array}{|c|l|l|} \hline \text { Enzyme } & \text { Prokaryotes/Eukaryotes/Both } & \text { Function } \\ \hline \text { Helicase } & & \\ \hline \begin{array}{c} \text { Single-stranded DNA-binding } \\ \text { protein } \end{array} & & \\ \hline \text { Primase } & & \\ \hline \text { DNA polymerase III } & & \\ \hline \text { DNA polymerase } \boldsymbol{\alpha} & & \\ \hline \text { DNA polymerase I } & & \\ \hline \end{array} $$ $$ \begin{array}{|c|l|l|} \hline \text { Enzyme } & \text { Prokaryotes/Eukaryotes/Both } & \text { Function } \\ \hline \text { RNase H } & & \\ \hline \text { DNA ligase } & & \\ \hline \text { DNA topoisomerases } & & \\ \hline \end{array} $$

Problem 2

Compare and contrast heterochromatin and euchromatin based on the following characteristics: $$ \begin{array}{|c|c|c|} \hline \text { Characteristic } & \text { Heterochromatin } & \text { Euchromatin } \\ \hline \text { Density of chromatin packing } & & \\ \hline \text { Appearance under light microscopy } & & \\ \hline \text { Transcriptional activity } & & \\ \hline \end{array} $$

Problem 2

What does PCR accomplish for a researcher? What about Southern blotting? \- PCR: \- Southern blotting:

Problem 2

Between the leading strand and lagging strand, which is more prone to mutations? Why?

Problem 2

What are the base-pairing rules according to the Watson-Crick model?

Problem 3

During DNA sequencing, why does the DNA polymer stop growing once a dideoxyribonucleotide is added?

Problem 3

What are the three major structural differences between DNA and RNA? 1. 2. 3

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