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Buffers that are based on 3-morpholinopropanesulfonic acid (MOPS) are often used in RNA analysis. The useful pHrange of a MOPS buffer is 6.5 to role="math" localid="1663323468024" 7.9. Estimate the role="math" localid="1663323276603" Kaof MOPS.

Short Answer

Expert verified

The value of MOPS is6×10-8.

Step by step solution

01

Definition of Concept.

Capacity: The amount of heat energy required to raise the temperature of a body by a certain amount is known as heat capacity. The amount of heat in joules required to raise the temperature 1 Kelvin is known as heat capacity (symbol: C) in SI units.

02

Calculate the Ka of MOPS.

Considering the given information:

The pH range of 3-morpholinepropanesulfonic acid (MOPS) is 6.5to7.9.

From the given pH range, calculate the average role="math" localid="1663323531566" pKa.

Average role="math" localid="1663323618817" pKa(center of range)

=(7.9+6.5)/2=7.2

Calculate the role="math" localid="1663323643490" Kavalue from pKavalue,

AveragepKa=7.2

Ka=10-7.2=6.3095734×10-8=6×10-8.

Therefore, the Kavalue of MOPS is 6×10-8.

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