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In 'EcoRI' 'co' stands for (a) Genus (b) Species (c) Strain (d) Restriction enzyme

Short Answer

Expert verified
'co' in 'EcoRI' stands for 'coli', which is the species.

Step by step solution

01

Understanding the Nomenclature of Restriction Enzymes

Restriction Enzymes are typically named using a system of abbreviation. The first letter of the name denotes the genus of the prokaryotic organism from which the enzyme was isolated, and the next two letters denote the species. For example, in the restriction enzyme 'EcoRI', 'E' stands for Escherichia (the genus) and 'co' stands for coli (the species).
02

Identify what 'co' stands for

Using the rule stated in the previous step, forming the name using the genus and species of the prokaryotic organism, we can infer that 'co' in 'EcoRI' stands for 'coli', which is the species of the prokaryotic organism.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

EcoRI
Enzymes are fascinating tools, especially in molecular biology where they serve as precise biological scissors. EcoRI is one such enzyme, used extensively to cut DNA at specific sites. It belongs to a class known as restriction enzymes. Interestingly, the name 'EcoRI' is derived from the organism from which it was first isolated. The 'E' represents the genus Escherichia, while 'co' refers to the species coli.

The 'RI' in its name indicates the particular strain or order of discovery ('R' often refers to the strain, and 'I' stands for first). Enzymes like EcoRI are invaluable in genetic engineering, allowing scientists to splice DNA and understand genetic sequences by targeting specific sequences to cut, known as palindromic sites. In the case of EcoRI, it recognizes the palindromic sequence 'GAATTC' and makes a clean cut between 'G' and 'A', which allows for DNA fragments to be manipulated with great precision.
Prokaryotic Organism
Prokaryotic organisms are the simplest forms of life, and with this simplicity comes their significance in the world of science. They are unicellular and do not have a nucleus or other membrane-bound organelles. Bacteria and archaea are prominent examples of prokaryotes, and they are found in virtually every environment on Earth. Their genetic material is a single circular DNA molecule that floats freely within the cell.

Importance in Research

Prokaryotes are critical to genetic engineering and biotechnology. Many of the tools used in these fields, like restriction enzymes, are derived from prokaryotes. Their simple genome and rapid reproduction make them ideal for genetic studies and the production of substances like insulin and antibiotics. Moreover, prokaryotic organisms are crucial for understanding basic biological processes and the development of new technologies.
Genus and species
Understanding the biological classification system is essential for grasping complex scientific concepts. At the heart of this system are the categories 'genus' and 'species'. The genus is a group of related species that share common characteristics and are closely related, while the species is the basic unit of biological classification and a group of organisms that can interbreed and produce fertile offspring.

Classification Foundation

For example, in the restriction enzyme's name 'EcoRI', 'Eco' stems from the taxonomic nomenclature, where 'E' corresponds to the genus Escherichia, and 'co' ties to the species coli. This precise naming allows researchers across the globe to communicate unambiguously about the organisms they study, ensuring consistency and clarity in scientific research and documentation.

The Linnaean system, developed by Carl Linnaeus, is the foundation for this classification structure and remains a cornerstone of biological nomenclature and organization. By using this system, scientists can not only name organisms but also predict properties and the relationships among different organisms.

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Most popular questions from this chapter

Select the incorrect statement. (a) More than 900 restriction enzymes have been isolated from over 230 strains of bacteria. (b) In the year 1963 , the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated. (c) Some key tools for recombinant DNA technology are restriction enzyme, polymerase enzyme, ligase, vectors and host root organisms. (d) EcoRI cut the DNA between bases \(\mathrm{A}\) and \(\mathrm{T}\) only when the sequence GAATTC is present in the DNA.

Which of the following antibiotic resistance genes are used as selectable marker for \(\mathrm{E}\). coli? (a) Chloramphenicol (b) Tetracycline (c) Kanamycin (d) All of these

Transgenic plants are produced by inserting desired genes in (a) pBR322 (b) \(\mathrm{T}_{\text {i }}\) plasmids (c) Lambda phage (d) None of these

Select the incorrect matching. (a) pBR322 \(-\mathrm{E}\). coli cloning vector (b) EcoRI, Cla I, - Restriction enzyme Hind III (c) ROP \(\quad\) - Protein involved in the replication of the plasmid (d) \(\mathrm{PCR} \quad-\) Technique in which multiple copies of the gene (or DNA) of interest is synthesized in vitro

Select the incorrect statement. (a) Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure. (b) Insertional inactivation of \(\beta\) -galactosidase leads to colourless colonies. (c) Insertional inactivation of \(\beta\) -galactosidase leads to blue color colonies. (d) In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme \(\beta\) -galactosidase.

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