Chapter 14: Problem 18
Translational control is usually aimed at preventing the initiation of translation. With regard to cellular efficiency, why do you think this is the case?
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Chapter 14: Problem 18
Translational control is usually aimed at preventing the initiation of translation. With regard to cellular efficiency, why do you think this is the case?
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What is antisense RNA? How does it affect the translation of a complementary mRNA?
As described in Chapter 13, enzymes known as aminoacyl-tRNA synthetases are responsible for attaching amino acids to tRNAs. Let's suppose that in a mutant bacterium tryptophanyl-tRNA synthetase has a reduced ability to attach tryptophan to tRNA: its activity is only \(10 \%\) of that found in a normal bacterium. How would attenuation of the \(\operatorname{trp}\) operon be affected? Would the operon be more or less likely to be attenuated? Explain your answer.
How are the actions of lac repressor and trp repressor similar and how are they different with regard to their binding to operator sites, their effects on transcription, and the influences of small effector molecules?
If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?
Would a mutation that inactivated lac repressor and prevented it from binding to the lac operator site result in the constitutive expression of the lac operon under all conditions? Explain. What is the disadvantage to the bacterium of having a constitutive lac operon?
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