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Chapter 10: Problem 260

How are genes transferred in bacteria?

Short Answer

Expert verified
Genes are transferred in bacteria through three main methods: conjugation, transformation, and transduction. Conjugation involves the exchange of genetic material between two bacterial cells via a pilus, with the donor cell containing a fertility plasmid. Transformation occurs when a bacterial cell takes up exogenous DNA from its environment, often from lysed cells, and incorporates it into its chromosome. Transduction is the process of gene transfer facilitated by bacteriophages, which mistakenly package bacterial DNA inside their capsids and inject it into recipient cells upon infection.

Step by step solution

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1. Conjugation

Conjugation is a process where two bacterial cells temporarily join together and exchange genetic material through a connection known as a pilus. One of the bacteria, called the donor, contains a special circular piece of DNA called the F (fertility) plasmid. The F plasmid carries genes for synthesizing the pilus and is self-transmissible. During conjugation, the donor bacteria transfers a copy of its F plasmid to the recipient bacteria, which allows the recipient to synthesize its own pilus. Once the recipient receives the F plasmid, it also becomes a potential donor, enabling it to share the plasmid with other bacteria cells.
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2. Transformation

Transformation is a process where a bacterial cell takes up exogenous genetic material from its environment. This typically involves the uptake of naked DNA fragments released by other bacteria that have lysed (broken open). The recipient bacterium incorporates the foreign DNA into its own chromosome via recombination. This integration can result in the addition of new genetic elements, such as antibiotic resistance genes, or it can replace existing genes by homologous recombination. This transfer of genetic material plays a significant role in the evolution and adaptation of bacteria.
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3. Transduction

Transduction is a process where genes are transferred from one bacterium to another by a bacteriophage, which is a virus that infects bacteria. In this process, the bacteriophage mistakenly packages a fragment of the host bacterium's DNA into its viral capsid (protein coat) instead of its own viral DNA. When the bacteriophage infects a new bacterial cell, it injects the mistakenly packaged fragment of bacterial DNA into the recipient cell, which can then be incorporated into the recipient's chromosome via recombination. This type of gene transfer can result in the transmission of new genetic elements, such as virulence or antibiotic resistance genes, between different bacterial strains or species.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

Conjugation in Bacteria
Conjugation in bacteria is like bacterial "dating," where two cells come together and exchange genetic material. This process relies on a physical connection between the cells, called a pilus. Imagine a bridge that allows bacteria to share their secrets! One bacterium, the donor, has a special piece of DNA called the F (fertility) plasmid. This plasmid isn't just any DNA; it holds the instructions for building the pilus bridge.
  • The donor bacterium uses the pilus to connect with a recipient bacterium.
  • The F plasmid travels across this bridge from the donor to the recipient.
  • Once the recipient receives the F plasmid, it can now make its own pilus and become a donor, spreading the genetic material further.
This fascinating method of gene transfer is a way bacteria can rapidly adapt to new environments and share important survival skills, like antibiotic resistance.
Bacterial Transformation
Bacterial transformation is akin to bacteria picking up and using lost pieces of DNA scattered in their environment. Occasionally, bacteria break open, releasing bits and pieces of their DNA. Other bacteria snoop up these DNA fragments, incorporating them into their own genetic make-up.
  • The recipient bacterium captures the "naked" DNA floating around.
  • It integrates this external DNA into its own genome through a process called recombination.
  • This can lead to new traits, like antibiotic resistance, manifesting in the recipient bacterium.
The significance of transformation lies in its role in bacterial adaptation and evolution. Through transformation, bacteria can easily acquire beneficial genes, enhancing their survival in various environments.
Transduction Process in Bacteria
The process of transduction is an extraordinary bacterial "package delivery" via their tiny viral companions, bacteriophages. These are viruses that specifically target bacteria. Sometimes during this process, bacteriophages pick up snippets of bacterial DNA by mistake instead of their own viral DNA.
  • The bacteriophage accidentally packages bacterial DNA into its viral capsid.
  • It carries this package to a new bacterium and injects the DNA into that cell.
  • The recipient bacterium may then incorporate this foreign DNA through recombination.
  • This can result in the sharing of many traits, like enhanced virulence or antibiotic resistance.
Transduction provides a means for genetic exchange and diversity among bacteria. It is a critical mechanism allowing bacteria to adapt rapidly to new challenges in their environment, including antibiotic attacks.

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Most popular questions from this chapter

The central dogma of biochemical genetics is the basic relationship between DNA, RNA, and protein. DNA serves as a template for both its own replication and for the synthesis of RNA, and RNA serves as a template for protein synthesis. How do viruses provide an exception to this flow scheme for genetic information?

Three \(E\). coli strains are given to you as a birthday present They are: 1) \(\left[\left(\mathrm{F}^{+}\right.\right.\) leu \(^{+}\) pro \(\left.-1\right) /\left(\right.\) leu \(^{+}\) pro \(\left.\left.-1\right)\right]\) (partially diploid for the two genes) 2) \(\mathrm{F}^{-}\) leu pro \(-2 \mathrm{Z}\) (lysogenic for the generalized transducing phage \(\bar{Z}\) ) 3) \(\mathrm{F}^{-}\) leut \(^{+}\) pro \(-1\left(\right.\) an \(\mathrm{F}^{-}\) derivative of strain 1 , having lost \(\mathrm{F}\) ) The strains either synthesize leucine (leu \(^{+}\) ) or do not and require it as a nutritional supplement (leu). All of the strains require proline. a) How could you determine whether pro \(-1\) and pro \(-2\) were alleles of the same gene? b) Suppose pro \(-1\) and pro \(-2\) are allelic, and the leu locus is contransduced with the pro locus. If you used phage \(Z\) to transduce genes from strain 3 to strain 2 , how would you determine the order of the leu, pro \(-1\), and pro \(-2\) markers?

How does the antibiotic streptomycin work?

Using a virus, how can one transform \(E\). coli bacteria unable to utilize galactose (gal mutants) into those that can utilize

How is a bacterial zygote different from a eukaryotic zygote?
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