Chapter 9: Problem 21
What is the physical state of DNA after being denatured by heat?
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Chapter 9: Problem 21
What is the physical state of DNA after being denatured by heat?
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Newsdate: March \(1,2030 .\) A unique creature has been discovered during exploration of outer space. Recently, its genetic material has been isolated and analyzed, and has been found to be similar in some ways to DNA in chemical makeup. It contains in abundance the 4 -carbon sugar erythrose and a molar equivalent of phosphate groups. In addition, it contains six nitrogenous bases: adenine(A), guanine(G), thymine(T), cytosine (C), hypoxanthine (H), and xanthine (X). These bases exist in the following relative proportion: \(A=T=H \quad\) and \(\quad C=G=X\) X-ray diffraction studies have established a regularity in the molecule and a constant diameter of about \(30 \AA\). Together, these data have suggested a model for the structure of this molecule. (a) Propose a general model of this molecule, and briefly describe it. (b) What base-pairing properties must exist for \(\mathrm{H}\) and for \(\mathrm{X}\) in the model? (c) Given the constant diameter of \(30 \AA\), do you think either (i) both \(\mathrm{H}\) and \(\mathrm{X}\) are purines or both pyrimidines, or (ii) one is a purine and one is a pyrimidine?
Adenine may also be named 6-amino purine. How would you name the other four nitrogenous bases, using this alternative system? (O is oxy, and \(\mathrm{CH}_{3}\) is methyl.)
One of the most common spontaneous lesions that occurs in DNA under physiological conditions is the hydrolysis of the amino group of cytosine, converting it to uracil. What would be the effect on DNA structure if a uracil group replaced cytosine?
You are provided with DNA samples from two newly discovered bacterial viruses. Based on the various analytical techniques discussed in this chapter, construct a research protocol that would be useful in characterizing and contrasting the DNA of both viruses. Indicate the type of information you hope to obtain for each technique included in the protocol.
List three main differences between DNA and RNA.
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