/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Problem 7 Restriction sites are palindromi... [FREE SOLUTION] | 91影视

91影视

Log out

Learning Materials

Features

Discover

Chapter 17: Problem 7

Restriction sites are palindromic; that is, they read the same in the \(5^{\prime}\) to \(3^{\prime}\) direction on each strand of DNA. What is the advantage of having restriction sites organized this way?

Short Answer

Expert verified
Answer: The advantage of palindromic restriction sites is that they allow restriction enzymes to recognize and bind to target sites on both strands of the DNA molecule, ensuring complete cleavage of both strands. This produces compatible sticky or cohesive ends that can be ligated with other DNA fragments sharing the same restriction site, making it easier for scientists to manipulate and join different DNA fragments in molecular biology and genetic engineering applications.

Step by step solution

01

1. Understanding restriction enzymes and their function

Restriction enzymes, also known as restriction endonucleases, are proteins that recognize and cut specific sequences of DNA called restriction sites. These enzymes play a crucial role in molecular biology, particularly for genetic engineering and recombinant DNA technology, as they enable scientists to manipulate and analyze DNA.
02

2. Explaining the structure of DNA and the \(5^{\prime}\) to \(3^{\prime}\) orientation

DNA is a double-stranded molecule consisting of two complementary strands running in opposite directions. Each strand has a \(5^{\prime}\) end (where the phosphate group is attached to the 5th carbon of the sugar ring) and a \(3^{\prime}\) end (where the hydroxyl group is attached to the 3rd carbon of the sugar ring). The strands are antiparallel, meaning one runs in the \(5^{\prime}\) to \(3^{\prime}\) direction while the other runs in the \(3^{\prime}\) to \(5^{\prime}\) direction.
03

3. Describing palindromic sequences in DNA

Palindromic sequences in DNA are sequences that read the same on both strands when read from the \(5^{\prime}\) to \(3^{\prime}\) direction. For example, if the \(5^{\prime}\) to \(3^{\prime}\) sequence in one strand is "GAATTC," the complementary strand will also read "GAATTC" in the \(5^{\prime}\) to \(3^{\prime}\) direction.
04

4. The advantage of palindromic restriction sites

Having restriction sites organized as palindromic sequences offers a significant advantage. Since restriction enzymes only recognize and bind to specific sequences, having palindromic sites allows the enzyme to recognize the target site on both strands of the DNA. This ensures that the enzyme can cut through both strands of the DNA molecule. This cleavage produces compatible sticky or cohesive ends that can be ligated with other DNA fragments sharing the same restriction site, making it easier for scientists to manipulate and join different DNA fragments.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with 91影视!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

How is fluorescence in situ hybridization (FISH) used to produce a spectral karyotype?

What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

Gene targeting and genome editing are both techniques for removing or modifying a particular gene, each of which can produce the same ultimate goal. Describe some of the differences between the experimental methods used for these two techniques.

What safety considerations must be taken before CRISPR-Cas is used to edit human embryos to cure disease?

List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
See all solutions

Recommended explanations on Biology Textbooks

Substance Exchange

Read Explanation

Biology Experiments

Read Explanation

Reproduction

Read Explanation

Responding to Change

Read Explanation

Heredity

Read Explanation

Cells

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.