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Problem 1

Which of the following cut(s) DNA at highly specific target sequences? a. DNA ligase b. DNA polymerase \(c_{\text {thestriction enzymes }}\) d. RNA polymerase

Problem 2

The propagation of recombinant DNA in a culture of \(E\). coli is known as a. \(\mathrm{PCR}\). b. DNA hybridization. c. DNA ligation. d. DNA cloning.

Problem 3

Genetic engineering a. can be used to make many copies of recombinant DNA introduced into a host cell. b. can be used to alter the inherited characteristics of an organism. c. raises ethical questions in the minds of some people. d. all of the above

Problem 4

Which of the following cell types displays the least developmental flexibility? a. zygote b. inner cell mass in a blastocyst c. bone marrow stem cell d. neuron

Problem 5

When DNA fragments are placed on an electrophoresis gel and subjected to an electrical current, which fragments move the farthest in a given time? a. the smallest b. the largest c. PCR fragments d. mRNA fragments

Problem 6

A short, single-stranded sequence of DNA whose bases are complementary to a portion of the DNA on another DNA strand is called. a. a DNA hybrid. b. a clone. c. a DNA probe. d. an mRNA.

Problem 7

Small loops of nonchromosomal DNA that are found naturally in bacteria are called a. plasmids. b. primers. c. amplimers. d. clones.

Problem 8

If the DNA sequences at the beginning and end of a gene are known, which of the following methods can be used to produce billions of copies of the gene in a few hours? a. RNAi b. reproductive cloning c. therapeutic cloning d. \(\mathrm{PCR}\)

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