Chapter 5: Problem 20
Are mitotic recombinations and sister chromatid exchanges effective in producing genetic variability in an individual? in the offspring of individuals?
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Chapter 5: Problem 20
Are mitotic recombinations and sister chromatid exchanges effective in producing genetic variability in an individual? in the offspring of individuals?
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In this chapter, we focused on linkage, chromosomal mapping, and many associated phenomena. In the process, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions? (a) How was it established experimentally that the frequency of recombination (crossing over) between two genes is related to the distance between them along the chromosome? (b) How do we know that specific genes are linked on a single chromosome, in contrast to being located on separate chromosomes? (c) How do we know that crossing over results from a physi- cal exchange between chromatids? (d) How do we know that sister chromatids undergo recombination during mitosis? (e) When designed matings cannot be conducted in an organism (for example, in humans), how do we learn that genes are linked, and how do we map them?
Colored aleurone in the kernels of corn is due to the dominant allele \(R\). The recessive allele \(r,\) when homozygous, produces colorless aleurone. The plant color (not the kernel color) is controlled by another gene with two alleles, \(Y\) and \(y\). The dominant \(Y\) allele results in green color, whereas the homozygous presence of the recessive \(y\) allele causes the plant to appear yellow. In a testcross between a plant of unknown genotype and phenotype and a plant that is homozygous recessive for both traits, the following progeny were obtained: $$\begin{array}{lc} \text { colored, green } & 88 \\ \text { colored, yellow } & 12 \\ \text { colorless, green } & 8 \\ \text { colorless, yellow } & 92 \end{array}$$ Explain how these results were obtained by determining the exact genotype and phenotype of the unknown plant, including the precise arrangement of the alleles on the homologs.
DNA markers have greatly enhanced the mapping of genes in humans. What are DNA markers, and what advantage do they confer?
Another cross in Drosophila involved the recessive, X-linked genes yellow \((y),\) white \((w),\) and \(c u t(c t) .\) A yellow-bodied, white-eyed female with normal wings was crossed to a male whose eyes and body were normal but whose wings were cut. The \(\mathrm{F}_{1}\) females were wild type for all three traits, while the \(\mathrm{F}_{1}\) males expressed the yellow-body and white- eye traits. The cross was carried to an \(\mathrm{F}_{2}\) progeny, and only male offspring were tallied. On the basis of the data shown here, a genetic map was constructed. (a) Diagram the genotypes of the \(\mathrm{F}_{1}\) parents. (b) Construct a map, assuming that white is at locus 1.5 on the X chromosome. (c) Were any double-crossover offspring expected? (d) Could the \(\mathrm{F}_{2}\) female offspring be used to construct the map? Why or why not?
In a series of two-point mapping crosses involving five genes located on chromosome II in Drosophila, the following recombinant (single-crossover) frequencies were observed: $$\begin{array}{lc} p r-a d p & 29 \% \\ p r-v g & 13 \\ p r-c & 21 \\ p r-b & 6 \\ a d p-b & 35 \\ a d p-c & 8 \\ a d p-r g & 16 \\ v g-b & 19 \\ v g-c & 8 \\ c-b & 27 \end{array}$$ (a) Given that the adp gene is near the end of chromosome II (locus 83 ), construct a map of these genes. (b) In another set of experiments, a sixth gene, \(d\), was tested against \(b\) and \(p r\) $$\begin{array}{ll} d-b & 17 \% \\ d-p r & 23 \% \end{array}$$ Predict the results of two-point mapping between \(d\) and \(c, d\) and \(v g,\) and \(d\) and \(a d p\)
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