Chapter 14: Problem 29
List as many different categories of protein functions as you can. Wherever possible, give an example of each category.
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Chapter 14: Problem 29
List as many different categories of protein functions as you can. Wherever possible, give an example of each category.
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In this chapter, we focused on the translation of mRNA into proteins as well as on protein structure and function. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions: (a) What experimentally derived information led to Holley's proposal of the two-dimensional cloverleaf model of tRNA? (b) What experimental information verifies that certain codons in mRNA specify chain termination during translation? (c) How do we know, based on studies of Neurospora nutritional mutations, that one gene specifies one enzyme? (d) On what basis have we concluded that proteins are the end products of genetic expression? (e) What experimental information directly confirms that the genetic code, as shown in Figure \(13-7,\) is correct? (f) How do we know that the structure of a protein is intimately related to the function of that protein?
Review the Chapter Concepts list on \(\mathrm{p} .371 .\) These all relate to the translation of genetic information stored in mRNA into proteins and how chemical information in proteins impart function to those molecules. Write a brief essay that discusses the role of ribosomes in the process of translation as it relates to these concepts.
Three independently assorting genes are known to control the following biochemical pathway that provides the basis for flower color in a hypothetical plant: Colorless \(\stackrel{A^{-}}{\longrightarrow}\) yellow \(\stackrel{B^{-}}{\longrightarrow}\) green \(\stackrel{C-}{\longrightarrow}\) speckled Three homozygous recessive mutations are also known, each of which interrupts a different one of these steps. Determine the phenotypic results in the \(\mathrm{F}_{1}\) and \(\mathrm{F}_{2}\) generations resulting from the \(P_{1}\) crosses of true-breeding plants listed here: (a) speckled \((A A B B C C) \times\) yellow \((A A b b C C)\) (b) yellow \((A A b b C C) \times \operatorname{green}(A A B B c c)\) (c) colorless \((a a B B C C) \times\) green \((A A B B c c)\)
Contrast the roles of tRNA and mRNA during translation and list all enzymes that participate in the transcription and translation process.
Knowing the quaternary structure of proteins, specifically their surface properties, provides novel opportunities for enhancing the development of targeted drug therapies. (a) Referring to Figure \(14-16\) and the accompanying discussion, predict the class(es) of amino acids likely to reside on the surface of a protein within a cell. (b) Assuming that Glu, Arg, and Asp exist in a functional site of a receptor molecule on the surface of a cell, provide an amino acid sequence that is likely to be effective in blocking that site. (c) The androgen receptor associated with prostate cancer has recently been targeted using synthetic molecules known generally as peptidomimetics, many of which incorporate D-amino acid stereoisomers rather than the more naturally occurring L-amino acid stereoisomers. Why might \(\mathrm{D}\) -amino acid stereoisomers be especially useful for inhibiting a functional site of the androgen receptor, or any deleterious functional site for that matter? (d) Rational drug design is a relatively recent approach to the design of targeted therapeutic agents. Predict how knowledge of protein structure, especially surface properties, might be used in rational drug design as compared with the more classical "trial-and-error" approach.
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