Chapter 13: Problem 16
Most proteins have more leucine than histidine residues, but more histidine than tryptophan residues. Correlate the number of codons for these three amino acids with this information.
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Chapter 13: Problem 16
Most proteins have more leucine than histidine residues, but more histidine than tryptophan residues. Correlate the number of codons for these three amino acids with this information.
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Present an overview of various forms of posttranscriptional processing in eukaryotes. For each, provide an example.
What was the initial evidence for the existence of mRNA?
In a mixed copolymer experiment, messages were created with either \(4 / 5 \mathrm{C}: 1 / 5 \mathrm{A}\) or \(4 / 5 \mathrm{A}: 1 / 5 \mathrm{C}\). These messages yielded proteins with the following amino acid compositions. Using these data, predict the most specific coding composition for each amino acid.
Sydney Brenner argued that the code was nonoverlapping because he considered that coding restrictions would occur if it were overlapping. A second major argument against an overlapping code involved the effect of a single nucleotide change. In an overlapping code, how many adjacent amino acids would be affected by a point mutation? In a nonoverlapping code, how many amino acid(s) would be affected?
Recent observations indicate that alternative splicing is a common way for eukaryotes to expand their repertoire of gene functions. Studies indicate that approximately 50 percent of human genes exhibit alternative splicing and approximately 15 percent of disease-causing mutations involve aberrant alternative splicing. Different tissues show remarkably different frequencies of alternative splicing, with the brain accounting for approximately 18 percent of such events (Xu et al., 2002 . Nuc. Acids Res. \(30: 3754-3766\) ). (a) Define alternative splicing and speculate on the evolutionary strategy alternative splicing offers to organisms. (b) Why might some tissues engage in more alternative splicing than others?
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