Chapter 12: Problem 14
A particular variant of the lambda bacteriophage has a DNA double-stranded genome of 51,365 base pairs. How long would this DNA be?
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Chapter 12: Problem 14
A particular variant of the lambda bacteriophage has a DNA double-stranded genome of 51,365 base pairs. How long would this DNA be?
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Supercoiled DNA is slightly unwound compared to relaxed DNA and this enables it to assume a more compact structure with enhanced physical stability. Describe the enzymes that control the number of supercoils present in the \(E .\) coli chromosome. How much would you have to reduce the linking number to increase the number of supercoils by five?
An article entitled "Nucleosome Positioning at the Replication Fork" states: "both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands" (Lucchini et al., 2002). Given this statement, how would one compare the distribution of nucleosomes and DNA in newly replicated chromatin? How could one experimentally test the distribution of nucleosomes on newly replicated chromosomes?
Describe the structure of giant polytene chromosomes and how they arise.
Review the Chapter Concepts list on \(\mathrm{p} .322 .\) These all relate to how DNA is organized in viral, prokaryote, and eukaryote chromosomes. Write a short essay that contrasts the major differences between the organization of DNA in viruses and bacteria versus eukaryotes.
A number of recent studies have determined that disease pathogenesis, whether it be related to viruses, cancer, aging, or a host of other causes, is often associated with specific changes in DNA methylation. If such patterns are to be considered as biomarkers for disease diagnosis what requisite criteria would you consider essential to their use?
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