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Chapter 9: Problem 2

Which of the following is LEAST likely to affect the purification of genomic DNA for analysis in molecular methods? a. Sample volume required for hybridization b. Size of the nucleic acid to be isolated c. Presence of RNA in the sample d. Presence of protein in the sample e. Presence of deoxyribose

Short Answer

Expert verified
e. Presence of deoxyribose

Step by step solution

01

- Understand the factors affecting DNA purification

When purifying genomic DNA, several factors can affect the process, such as contaminants (RNA, proteins), nucleic acid size, and sample requirements.
02

- Evaluate Sample Volume

Sample volume required for hybridization generally pertains to the application after purification. It does not directly affect the DNA purification process itself.
03

- Evaluate Nucleic Acid Size

The size of the nucleic acid to be isolated can influence the purification process techniques, as specific methods are tailored for different molecule sizes.
04

- Evaluate the Presence of RNA

RNA presence in the sample can contaminate genomic DNA during purification, requiring additional steps to remove it.
05

- Evaluate the Presence of Proteins

Proteins in the sample can also contaminate DNA solutions and need to be removed during the purification process.
06

- Evaluate the Presence of Deoxyribose

Deoxyribose is a component of DNA itself and is naturally present in the sample. Its presence does not affect the purification of genomic DNA.
07

- Identify the Least Affecting Factor

Based on the evaluations, the presence of deoxyribose (option e) is least likely to affect the purification of genomic DNA.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

headline of the respective core concept
Genomic DNA is the complete set of DNA in an organism, which includes all of its genes. This DNA carries hereditary information and is crucial for various biological processes. To analyze genomic DNA, researchers often need to purify it from other cellular components. The purification process is important because any contaminants can interfere with subsequent molecular methods. Contaminants such as proteins and RNA can be particularly troublesome. Understanding the nature of genomic DNA and the challenges in its purification is the first step in successful DNA analysis.
headline of the respective core concept
Nucleic acid contamination is a significant challenge in the purification of genomic DNA. Contaminants like RNA and proteins can interfere with the accuracy of molecular methods. RNA contamination is common because it is abundant in cells. Special enzymes, such as RNases, can be used to degrade RNA, ensuring that the genomic DNA sample is pure. Proteins can bind to DNA and hamper its isolation. Using proteases can break down these proteins, ensuring a cleaner DNA sample. To effectively purify genomic DNA, it's essential to remove these contaminants to prevent them from affecting downstream applications like PCR (Polymerase Chain Reaction) or sequencing.
headline of the respective core concept
Molecular methods are techniques used in biology to study and manipulate molecules like DNA, RNA, and proteins. These methods include PCR, sequencing, and hybridization. The purity of genomic DNA is crucial for the success of these methods. Contaminants can inhibit enzymes used in PCR or interfere with the accuracy of sequencing results. Therefore, an essential step in molecular analysis is ensuring that the DNA is free from impurities. By understanding molecular methods, researchers can better appreciate the importance of DNA purification in obtaining reliable experimental results.
headline of the respective core concept
Hybridization sample volume refers to the amount of DNA needed for hybridization techniques, such as microarray analysis. While the volume of DNA required is crucial for the experiment itself, it does not impact the DNA purification process directly. DNA purification focuses on isolating the DNA free from contaminants. Once purified, the DNA can be quantified and then used in the necessary volume for hybridization. Thus, while sample volume is an important consideration for downstream applications, it is not a factor that affects the purification of genomic DNA.

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Most popular questions from this chapter

Why is it important to add RNase to the patient sample when isolating genomic DNA? a. RNase serves as an enzymatic tag on DNA probes. b. RNase will remove excess collagen from the patient sample. c. RNase will transport the amino acid to the ribosome, where the peptide chain elongates. d. RNase clones DNA to RNA. e. RNase digests RNA in the patient sample that could copurify with genomic DNA.

All of the following describe a nucleic acid probe except: a. Short strand of DNA of a known sequence b. Short strand of RNA of a known sequence c. Can be cloned in bacteria d. Can be inserted into a plasmid vector e. Can be labeled with avidin
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