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DNA is the main hereditary material inherited to the next generations and maintains cell integrity while dividing.Its content changes with the events occurring in the cells like the S phase, mitosis, and meiosis. It is the main ingredient that is distributed to the daughter cells in the form of chromosomes.

The cell cycle is controlled by some specific cytoplasmic molecules that signal the different phases of the cell cycle.

All events in the cell cycle occur in a sequence that is due to a control system of the cell cycle involving molecules that work cyclically to coordinate all events of the cell cycle. There are three main checkpoints in the cell cycle that are controlled by these molecules.

In experiment 2, the cell of G1 is fused with the cell in the dividing phase. The resulting cell of G1 initiated division due to the influence of cell cycle control molecules, and it skipped the step of DNA replication in the S phase.

So the DNA is not doubled in this cell, but the cell that was in the M phase from initiation contains a doubled amount of DNA compared to the cell that started division after fusion. So the resulting nuclei have different amounts of DNA.