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The proteins are the long polypeptide chains that consist of amino acid sequences. The structure of amino acids consists of amino terminals as well as carboxy-terminal.

Intercellular adhesion complexes that are semi-permeable in property are known as tight junctions.The proteins that are present in these junctions are known as tight junction proteins.

The proteins are involved in the regulatory function that facilitates the movement of ions, water, and solutes.

The amino acids present in the tight junction protein have polar as well as non-polar ends.The polar part of this protein faces the aqueous layer, and the non-polar part is found inside the membrane.

By comparing with figure 5.14, the location of polar amino acids is seen in the tail region(end), cytoplasmic loop, and the extracellular loops. The non-polar amino acids are located in the four membranes that are present in between the tail and the loop region.