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Eukaryotes require an RNA polymerase to bind to a DNA sequence upstream of a gene in order to initiate transcription of that gene, just like prokaryotic organisms do. In contrast to prokaryotic organisms, eukaryotic RNA polymerase requires extra proteins, or transcription factors, to help transcription begin.

Transcription can only begin once RNA polymerase has bound. In eukaryotic cells, RNA polymerase cannot start transcription on its own. Eukaryotic transcription is regulated by two different categories of transcription factors: To help RNA polymerase bind to the core promoter region, general (or basal) transcription factors attach to the area. specific transcription factors interact with the proteins in the core promoter area by binding to different locations outside of the core promoter region to increase or repress the activity of the polymerase.

Therefore, other options such as a protein, DNA polymerase and a transcription factor became irrelevant as Transcription can only begin once RNA polymerase has bound.