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91Ó°ÊÓ

A researcher wants to study cancer cells from a patient with breast cancer. Is cloning the cancer cells an option?

Short Answer

Expert verified

Cellular cloning of the breast cancer cells will establish a cell line, which can be used for further analysis

Step by step solution

01

Molecular Cloning

A specific sequence in a DNA sample can be separated by scientists using Southern blotting.

Steps of southern blotting:

1. Purified DNA fragments are transferred from a gel to a nylon membrane.

2 The fragments are incubated with a DNA probe complementary to the target sequence.

On the gel, RNA rather than DNA is employed for Northern blotting. In Western blotting, researchers put proteins into a gel and use antibodies to find them.

02

Cloning cancer cells 

Biomarkers should be used with cancer cells during cloning for better studies.

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